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KMID : 1025520090510050387
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Journal of Animal Science and Technology
2009 Volume.51 No. 5 p.387 ~ p.394
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Screening and Isolation of Chitinase and Chitosanase Producing Microbes from the Feces of Korean Native Calves Medicated DFMs Including Chitin
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Kim Tae-Il
Kwon Eung-Gi
Kim Hyeong-Cheol
Cho Young-Moo
Park Byung-Ki
Lee Won-Kyu
Im Seok-Ki
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Abstract
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This study was carried out to screen and identify the chitinase and chitosanase producing microorganisms from the feces of calves medicated DFM sincluding chitin in order to do the immune fortification of Korean Native calves.
Ten isolates were grown in the medium containing chitin and chitosan that had more than 105 cfu/g in feces. Among these 10 strains, 2 strains(HANDI 110 and HANDI 309) had the chitinase activities and 2 strains(HANWOO and HANYOO) had the chitosanase activities in calves’ feces. They showed no reaction in hemolysis tests by utilizing chitin and chitosan. The results from morphological, physicochemical and genetical identification indicated the HANDI 110 as a strain of Escherichia fergusonii, HANDI 309 was identified as a strain of Acinetobacter parvus, HANWOO was identified as a strain of Comamonas koreensis, and HANYOO as a strain of Chryseobacterium indologenes.
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KEYWORD
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Korean Native Calves, Chtinase, Chitosanase, Microbes, Feces, DFMs
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